Washington University School of Medicine - Cardiovascular Division - Center for Cardiovascular Research
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Dana
R. Abendschein, Ph.D. Bio Sketch Current Research Recent Publications Research Support
Associate Professor of Medicine, Cell Biology & Physiology, Assistant Vice-Chancellor and Assistant Dean for Animal Affairs, Washington University School of Medicine, St. Louis, MO Clinical
Sciences Research Bldg. S.U.N.Y.
at Fredonia, Fredonia, New York, B.S., Biology, 1974 1978 -1981: Postdoctoral Research Fellow, Cardiovascular Research Institute, University of California, San Francisco, CA Awards and Professional Activities: Beta
Beta Beta Research in this physiology laboratory focuses on the molecular responses of the arterial wall and heart to injury. We have shown that inhibition of coagulation proteases including factor Xa and tissue factor/factor VIIa complex are particularly effective to attenuate thrombosis and neointimal thickening after angioplasty-like injury in experimental animals. Current studies are designed to determine the time course and mechanisms of procoagulant activity on the surface of the injured vessel. Another aspect of this research has been to establish a reproducible preparation of angioplasty-like overstretch injury in the carotid arteries of mice to address the role of signaling pathways involved in stimulating vascular smooth muscle cells to proliferate and migrate into the developing neointima. Current studies are designed to determine the effect of knockouts of integrin receptors, G-proteins, and inflammatory receptor proteins on neointimal thickening after arterial injury. We are also interested in the role of inflammatory cells in inducing a more robust neointimal response after angioplasty in the diabetic state. Recently, we have implemented an isolated, working mouse heart preparation to study the role of calcium-independent phospholipase A2 and peroxisomes in diabetic cardiomyopathy. Trainees in the lab will be responsible for conducting hands-on experiments in intact animals, isolated vessels and hearts; assays of protein levels, enzymatic activities, or substrate utilization in samples of tissue; analysis of data; and composition of results of studies in presentation and manuscript formats. Oltrona L, Speidel CM, Recchia D, Wickline SA, Eisenberg PR, Abendschein DR: Inhibition of tissue factor-mediated coagulation markedly attenuates stenosis after balloon-induced arterial injury in minipigs. Circulation 1997;96:646-652. Ghigliotti G, Waissbluth AR, Speidel C, Abendschein DR, Eisenberg PR: Prolonged activation of prothrombin on the vascular wall after arterial injury. Arterioscl. Thromb. Vasc. Biol. 1998;18(2):250-257. St. Pierre J, Yang L-Y, Tamirisa K, Scherrer D, De Ciechi P, Eisenberg P, Tolunay E, Abendschein D: Tissue factor pathway inhibitor attenuates procoagulant activity and upregulation of tissue factor at the site of balloon-induced arterial injury in pigs. Arterioscl. Thromb. Vasc. Biol. 1999;19(9):2263-2268. Han X, Girard TJ, Baum P, Abendschein DR, Broze Jr GJ: Structural requirements for TFPI-mediated inhibition of neointimal thickening after balloon injury in the rat. Arterioscl. Thromb. Vasc. Biol. 1999;19:2563-2567. Abendschein DR, Baum PK, Martin DJ, Vergona R, Post J, Rumennik G, Sullivan ME, Eisenberg PR, Light DR: Effects of ZK-807834, a novel inhibitor of factor Xa on arterial and venous thrombosis in rabbits. J. Cardiovasc. Pharmacol. 2000;35:796-805. Han X, Abendschein DR, Kelley JG, Gross RW: Diabetes-induced changes in specific lipid molecular species in rat myocardium: Insulin-remediable and -nonremediable alterations. Biochem. J. 2000;352:79-89. Abendschein DR, Baum PK, Verhallen P, Eisenberg PR, Sullivan ME, Light DR: A novel synthetic inhibitor of factor Xa decreases early reocclusion and improves 24-h patency after coronary fibrinolysis in dogs. J. Pharmacol. Exp. Ther. 2001;296:567-572. Lanza GM, Yu X, Winter PM, Abendschein DR, Karukstis KK, Scott MJ, Fuhrhop RW, Scherrer DE, Wickline SA: Targeted antiproliferative drug delivery to vascular smooth muscle cells with an MRI nanoparticle contrast agent: Implications for rational therapy of restenosis. Circulation 2002;106:2842-2847. Abendschein DR, Yang LY, Chun J, Cho D, Scherrer D, St. Pierre J: Prolonged procoagulant activity on overstretch-injured coronary arteries in pigs. J. Thromb. Haemost. 2003;1:836-842. Ongoing
Research Support To develop a site-directed contrast agent that is broadly applicable for ultrasonic, magnetic resonance, and nuclear imaging of molecular epitopes. NIH R01 HL48593-05
04/01/02 - 03/31/07 To determine the role of PPAR /PGC-1 in myocardial metabolism during hypertrophic growth NIH R01 HL41250-10
04/01/02 - 03/31/07 To determine the role of calcium-independent phospholipase A2 on vascular smooth muscle cell tone, proliferation, and migration. NIH PPG2
P01 HL57278-06AI To simulate in animals the pathophysiology of diabetes in humans and to characterize the functional, metabolic, and morphologic phenotypes of transgenic animals needed to bridge fundamental metabolic and genetic insights with the pathogenesis of the disease. Berlex Biosciences To determine
the effect of anti-tissue factor-thrombomodulin fusion protein on coagulation
and thrombosis.
Center
for Cardiovascular Research
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